Class Category: Basic. The student should be capable of starting up, running daily QC and interpreting basic sample screening on the Flow Cytometer by the end of the course.

Requirements: Microsoft Office Power Point 2010 or later, computer speakers, email address, Skype address.

Text: Who Classification of Tumours of Haematopoietic and Lymphoid Tissue.  4th Edition, ISBN 9789283224310 (Not included in the cost of the classTo be ordered seperately)

Hours: 60-62 hrs of study divided between lessons, reading, quizzes, skype conference and final assignment. Approved by CMLTO (College of Medical Laboratory Technologists of Ontario) as a refresher course.

Course Outline:
          Section A:
 

1. Introduction to Flow Cytometry.
2. Definition of Flow Cytometry and how it works.
3. Types of samples used.
4. Fluidics in Flow Cytometry.
5. Optics in Flow Cytometry.
6. Photo Detectors and Electronics in the Flow.
7. Linear and Log amplification.
8. Basic Cell separation using Forward Scatter, Side Scatter and Fluorescence.
9. Color compensation what it is and why it is important.
10. Interpretation and Analysis.
11. Some gating strategies.
12. Reading list.
13. Quiz.
    
    Section B:

1. QC for Flow Cytometry and why it is done.
2. Linear Verification: set up and reading.
3. Flow Cell Alignment: set up and reading.
4. Setting and checking the discriminator.
5. Setting and checking Voltage alignment and fluorescence alignment.
6. Setting up and running color compensation.
7. Setting up sample controls.
8. Reagent QC.
9. Quiz.
   
   Section C:

1. Immunophenotyping: what it is, markers or antibodies used.
2. What are Cluster Designations (CD): list of some basic CD markers defined.
3. Sample setup, types of samples that can be used.
4. Concept of single marker, or antibody probe and multiple markers used in testing.
5. Sample setup.
6. Gating strategies.
7. Quiz.
   
   Section D

1. Criteria for diagnosis.
2. Basic testing Algorithms.
3. Normal Ranges in Peripheral Blood.
4. Disease States.
5. Final patient based assignment.

Instructor name: Beverley Rostant
Location: Online
Cost: $600.00 CAN (HST is included in the cost)

If required, payments can be made in two installments. The second payment must be received within 30 days of registration. This method of payment must have the pre-approval of the Instructor. Please contact

Payment can be made by email transfer in Canada only, check, postal money order or western union to:

Beverley Rostant
1704 Pine Grove Rd .
Marlbank, ON
K7K 2L0

If western union is used please send the western union order number to me by email info@immunologycourses.ca
Classes will be sent once payment is received.

Cancellation Policy: Please Read

Start Date:    Ongoing flexible start date.
End Date:     Ongoing until finished. Quizzes must be passed before the next section is sent out.
Time:            Tuesdays

Class Category: Intermediate The student should be capable of identifying and recognizing problems on the Flow Cytometer by the end of the course.

Requirements: Adobe Acrobat Reader

Text: None

Hours: 6 hrs

Course Outline:            

1. Sample troubleshootong.
2. QC troubleshooting.
3. Machine troubleshooting.

Instructor name: Beverley Rostant
Location: Online
Cost: $65.00 CAN (HST is included in the cost)

Payment can be made by email transfer in Canada only, check, postal money order or western union to:

Beverley Rostant
1704 Pine Grove Rd .
Marlbank, ON
K7K 2L0

If western union is used please send the western union order number to me by email info@immunologycourses.ca
Classes will be sent once payment is received.


Start Date:    Ongoing flexible start date.

Class Category: Basic. The student should be capable of understanding the basics of what Flow Cytometry is and what it tests.

Requirements: Microsoft Office Power Point 2010 or later, computer speakers, email address.

Course Outline:
         

1. Introduction to Flow Cytometry.
2. Definition of Flow Cytometry and how it works.
3. Types of samples used.
4. Basic Cell separation using Forward Scatter, Side Scatter and Fluorescence.
5. Color compensation what it is and why it is important.
6. Interpretation and Analysis.
7. Some gating strategies.
8. Immunophenotyping: what it is, markers or antibodies used.
9. What are Cluster Designations (CD): list of some basic CD markers defined.
10. Sample setup, types of samples that can be used.
11. Concept of single marker, or antibody probe and multiple markers used in testing.
12. Criteria for diagnosis.
13. Basic testing Algorithms.
14. Disease States.

Instructor name: Beverley Rostant
Location: Online
Cost: $65.00 CAN (HST is included in the cost)

Payment can be made by email transfer in Canada only, check, postal money order or western union to:

Beverley Rostant
1704 Pine Grove Rd .
Marlbank, ON
K7K 2L0

If western union is used please send the western union order number to me by email info@immunologycourses.ca
Classes will be sent once payment is received.

Start Date:    Ongoing flexible start date.